Zenit Science offers
Our commitment to quality ensures reliable and reproducible data generated by experienced molecular biology scientists.
All assays are optimized to fit microtiter formats, requiring only low amounts (0.1 ml) of your precious samples material.
When choosing our standard package solutions prices are readily visible in the ordering system.
We ensure a rapid turnaround to faciliate efficient identification of promising leads early in drug development – One week following compound delivery.
Cost-effective standard packages can be directly ordered using our online system. We also offer tailoring of assays according to specific customer needs.
Standard Hemolysis Assay
Standard assay includes assessment of hemolytic potential against human red blood cells at four different test compound concentrations (two-fold serial dilutions). The hemolysis ratio is determined by normalization against negative (no hemolysis) and positive (100% hemolysis) internal control samples. All samples and controls are run in triplicate to ensure reliability and statistical power.
Customized Hemolysis Assay
Zenit Science can perform specialized studies based on particular sample requirements or other customer needs. Please reach out to us by using the below form. As necessary we will organize a meeting to clarify details or better understand your requirements before we put together a formal written offer.
Hemolysis assay details
The in vitro hemolysis assay is a method used to assess the cytotoxicity of substances by measuring their ability to cause membrane lysis (breakdown) of red blood cells (RBCs). The assay is useful for indication of possible adverse effects on mammalian membranes early in drug development. Assessment of hemolytic potential is recommended by the US FDA for all excipients intended for injectable use.
Zenit Science offers a standard assay which includes assessment of hemolytic potential at four different test compound concentrations. The hemolysis ratio is determined by normalization against negative (no hemolysis) and positive (100% hemolysis) internal control samples. All samples and controls are run in triplicate to ensure reliability and statistical power.
We also offer customization of the assay according to specific customer needs or compound requirements.
Graph showing optical density (OD) measurements of more than 80 separate samples of red blood cells suspended in PBS. The raw data underscores the consistency and superior quality of red blood cells utilized in hemolysis assays conducted by Zenit Science.